A rapid chemical method for lysing Arabidopsis cells for protein analysis

Table 1 Chelators used in this study

*¹ Chelate-stability constants, which are available in the Dojindo website http://www.dojindo.co.jp/.
*² A 7-day-old Arabidopsis seedling was boiled for 10 min in the 50 - l lysis solution (100 mM Tris-HCl, pH 8.0, 1% w/v SDS and 20 or 50 mM chelator listed). Samples were regarded as lysed (+) or not (-), based on the development of green color of the chlorophyll in the solution.

ISSN: 1746-4811