Skip to main content

Table 1 Chelators used in this study

From: A rapid chemical method for lysing Arabidopsis cells for protein analysis

   Cell lysis*2
Chelator logK CaL * 1 20 mM 50 mM
EDTA 10.96 + +
EGTA 11 + +
NTA 6.41 - +
TTHA 10.06 - +
DTPA 10.74 - +
CyDTA 12.5 ± +
BAPTA 6.97 + +
  1. *1 Chelate-stability constants, which are available in the Dojindo website http://www.dojindo.co.jp/.
  2. *2 A 7-day-old Arabidopsis seedling was boiled for 10 min in the 50 - l lysis solution (100 mM Tris-HCl, pH 8.0, 1% w/v SDS and 20 or 50 mM chelator listed). Samples were regarded as lysed (+) or not (-), based on the development of green color of the chlorophyll in the solution.