Figure 8

Application of the chemical cell lysis method to various tissues of Arabidopsis (A) and to leaves of other plant species (B). (A) Indicated tissues of Arabidopsis (0.1-0.5 g/ml in concentrations) were subjected to the chemical lysis described in Figure 5 and then to SDS-PAGE. Proteins were visualized by CBB staining. The arrow head indicate the position of Rubisco large subunit (RbcL). (B) Protein samples were prepared by the method described in Figure 5 from leaves of mulberry (Morus bombycis), fig (Ficus carica), peach (Amygdalus persica) and downy cherry (Prunus tomentosa) as well as from leaves of Arabidopsis. Plants were lysed in approximately 0.25 g/ml concentrations. The samples were subjected to SDS-PAGE followed by CBB staining. The arrowhead indicates the position of RbcL.