Figure 3
From: A rapid chemical method for lysing Arabidopsis cells for protein analysis
Effects of cations on EDTA/EGTA-dependent Arabidopsis cell lysis. (A) A 7-day-old Arabidopsis seedling was put in the lysis solution (50 mM EDTA, 1% w/v SDS, 100 mM Tris-HCl, pH 8.0, and the indicated concentrations of NaCl, MgCl2 or CaCl2), and incubated at 100°C for 10 minutes. (B) Enlarged images of those shown in panel A.