Figure 2From: A rapid chemical method for lysing Arabidopsis cells for protein analysisEDTA/EGTA-dependent Arabidopsis cell lysis. A 7-day-old Arabidopsis seedling was placed in the lysis solution (100 mM Tris-HCl, pH 8.0, and the indicated concentrations of SDS and EDTA/EGTA), incubated at 65°C for 60 minutes, and then at 100°C for 5 minutes. Photographs were taken at the indicated time points.Back to article page