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Table 4 Pilot assay on a sub-set of M2 lines and estimation of the mutation frequencies.

From: sunTILL: a TILLING resource for gene function analysis in sunflower

Gene

Fragment size (bp)

Number of screened plants

Identified mutation

Mutation frequency*

   

total

missense

stop codon

silent

intronic

 

kasII

686

1152

4

0

1

0

3

1/168 kb

kasIII

1500

768

0

-

-

-

-

-

fad2-1

965

1152

3

1

-

1

1

1/332 kb

Plasmopara

1430

1152

2

-

-

-

2

1/766 kb

Total

4581

 

9

    

1/475 kb

  1. * To calculate the mutation frequency, the fragment size of each gene was reduced to 100 bp because of priming and PCR artefacts.