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Figure 7 | Plant Methods

Figure 7

From: A novel procedure for the quantitative analysis of metabolites, storage products and transcripts of laser microdissected seed tissues of Brassica napus

Figure 7

Transcript analysis. Closeness of expression signals obtained after microarray hybridisation of conventionally extracted total RNA and amplified RNA samples of conventional, column-based and bead-based extraction methods. (a &c) Electropherograms reflecting quality of total RNA, isolated with RNeasy® (a) and mRNA, isolated with Dynabeads® (c) from a 20 μm seed cryosection (43. DAF). (b &d) Electropherograms of mRNA from the same samples (a/c) after 2 rounds of amplification. (e) Portions of signal intensities obtained from each hybridisation and divided into six groups. Y-axis represent % of observation. (f) Scatterplots comparing log2-transformed means of expression signals from the different sample treatments. Means are filtered against low signal intensities (≤ 10) and high coefficients of variation (≥ 20%).

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Plant Methods

ISSN: 1746-4811