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Figure 4 | Plant Methods

Figure 4

From: A novel procedure for the quantitative analysis of metabolites, storage products and transcripts of laser microdissected seed tissues of Brassica napus

Figure 4

Metabolic intermediates in laser-microdissected tissues of B. napus measured by LC/MS. Levels of metabolites related to sugar breakdown (hexose-phosphates), glycolysis, citric acid (TCA) cycle and energy metabolism (nucleotides and pyrophosphate) were measured in LM-tissue of embryo (minimum tissue amount ~ 0.0063 mm3). 13C6-succinate was added prior extraction as internal standard. Data represent relative values and are given as mean +/- standard deviation (n = 3). Stars indicate statistically significant differences versus hypocotyl according to t-test (p < 0.05).

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Plant Methods

ISSN: 1746-4811