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Figure 2 | Plant Methods

Figure 2

From: A novel procedure for the quantitative analysis of metabolites, storage products and transcripts of laser microdissected seed tissues of Brassica napus

Figure 2

Total lipids in laser-microdissected tissues of B. napus measured by gas chromatography. (a) Typical chromatograms of lipid standard, LM-sample and blank; IS = internal standard added to tissue samples during extraction. (b) Fatty acids in LM-samples of hypocotyl, inner cotyledon and outer cotyledon of one dissected element per tissue type (minimum tissue amount ~0.0084 mm3). (c) Comparison of total lipids in hypocotyl, inner- and outer cotyledon tissue. (d &e) Tissue localisation within a cryosection and the adequate lipid distribution map (based on spin-echo NMR). Lipid levels are color-coded. Mean values +/- standard deviation are shown (n = 5). Stars in b and c indicate statistically significant differences versus hypocotyl according to t-test (p < 0.01).

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Plant Methods

ISSN: 1746-4811