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Figure 1 | Plant Methods

Figure 1

From: A novel procedure for the quantitative analysis of metabolites, storage products and transcripts of laser microdissected seed tissues of Brassica napus

Figure 1

Experimental workflow. Schematic overview of the laser-microdissection based analysis of metabolites, storage products and transcripts in seeds of Brassica napus: (I) Seeds are removed out of the silique and immediately snap-frozen in liquid nitrogen. Two seeds mounted for cryosectioning are cut in 20 μm thick cross sections. (II) Sections are transferred to PET membrane slides and freeze-dried for one week. (III) Tissues of interest are laser microdissected from the cross sections. Light microscopy pictures show a cryosection of a 28 day old rape seed (bar = 1 mm) (IV) Isolated tissues are processed in parallel for subsequent biochemical and transcript analyses. Abbreviations: hy-hypocotyl; ic-inner cotyledon; oc-outer cotyledon.

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Plant Methods

ISSN: 1746-4811