Table 1 Sequences of primers used for RT-PCR and qRT-PCR.
From: An effective virus-based gene silencing method for functional genomics studies in common bean
Primer name | Primer sequence (5→3, forward, reverse) | Protein name |
|---|---|---|
nod22 forw | ATGTTCCACAGTAGCAAAAACA | Nodulin 22 |
nod22 rev | TCACTGTACAAGCACAAGTCTC | Nodulin 22 |
nod22-ext. | GTCTTCGCCCGAAAAAGAAAGG GCAGAGGATTGCAACACCAAGA | Nodulin 22 |
nod22-int. | GAGACTGAGGGTATCGGCGACGTG GATTCTGGTAAACGGAACCTCCAC | Nodulin 22 |
PvSACPD-C' | TATGTACGACGGGGAAGACC GATTGGAGAAGCTGGAAGGT | Stearoyl-ACP desaturase (C-like) |
PvEf1-α | GGTCATTGGTCATGTCGACTCTGG GCACCCAGGCATACTTGAATGACC | Elongation factor-1 alpha |