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Table 2 Stability of the endogenous genes inserted into ALSV vectors in apple seedlings

From: Efficient virus-induced gene silencing in apple, pear and Japanese pear using Apple latent spherical virus vectors

  

No. of plants infected with ALSV maintained inserts/tested

ALSV vectors

Sizes of Inserts (bp)

1.5 mpi

3 mpi

6 mpi

rbcS201-ALSV

201

4/4

4/4

0/2a

rbcS102-ALSV

102

5/5

5/5

-a

rbcS51-ALSV

51

5/5

5/5

5/5

CPN60a-ALSV

201

11/12

7/12

7/12

actin-ALSV

201

5/5

5/5

3/5

EF-1a-ALSV

201

4/5

4/5

2/5

MdTFL-ALSV

201

3/3

3/3

3/3

Total (%)

 

37/39 (95)

33/39 (85)

20/32 (63)

  1. a Two plants with rbcS201-ALSV and 5 plants with rbcS102-ALSV died before 6 mpi.