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Figure 6 | Plant Methods

Figure 6

From: Efficient virus-induced gene silencing in apple, pear and Japanese pear using Apple latent spherical virus vectors

Figure 6

In situ hybridization analysis of apple seedlings infected with MdTFL-ALSV. In situ hybridization analysis of MdTFL1-mRNA and ALSV-RNAs in the apical tissues of the apple seedlings infected with wtALSV (A to C) or MdTFL-ALSV (D to I). Three RNA probes, i.e., antisense RNA (A, D, and G) or sense RNA (B, E, and H) to MdTFL1-mRNA, and complementary RNA to ALSV-RNA (C, F, and I). Arrows in (A) and (D) indicate the hybridization signals showing the expression of MdTFL1-mRNA. Arrows in (I) indicate the presence of virus in floral organs of the apple seedling infected with MdTFL-ALSV.

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