Figure 2

Proanthocyanidin accumulation in Lotus corniculatus leaves. A L. corniculatus leaf stained with DMACA (A-B). A L. corniculatus leaf stained with DMACA and embedded in LR White resin (C-D). A L. corniculatus leaf embedded in LR White resin without DMACA staining (E). Transverse section of a L. corniculatus leaf after DMACA staining and embedding in LR White resin (F-G). In Panel G, the cell walls have been stained with Toluidine Blue after sectioning of the samples shown in panel F. Transverse section of an unstained L. corniculatus leaf embedded in LR White resin without DMACA staining (H). cu-cuticle; sm-spongy mesophyll cells; va-vacuole; vb-vascular bundle. Bars represent 200 μm (A-E) and 50 μm (F-H).