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Table 4 Inheritance patterns of GFP in T1 progeny of four transgenic safflower lines

From: Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting

Transgenic line

Observed a

Expected b

Chi-square

P value

 

GFP+

GFP-

GFP+

GFP-

  

WT-TS-72

13

3

12

4

0.330

0.563

S-317-TS-57

17

7

18

6

0.220

0.637

S-317-TS-82

18

4

16.5

5.5

0.545

0.460

S-317-TS-88

33

13

32.5

13.5

0.026

0.871

  1. a GFP expression was scored using fluorescence microscopy on germinating seed.
  2. b Expected ratio if a dominant trait at a single locus is inherited by progeny (3:1 segregation ratio).