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Figure 4 | Plant Methods

Figure 4

From: De novo assembly of potential linear artificial chromosome constructs capped with expansive telomeric repeats

Figure 4

PCR analysis of the linear AC constructs. The primers specific to the left arm junction (L5 and L3) and right arm junction (R5 and R3) span the attL1 and attR1 sites, respectively. Lane 1: Left arm junction PCR using linear AC constructs capped with telomeric DNA at both ends. Lane 2: Right arm junction PCR using linear AC constructs capped with telomeric DNA at both ends. Lane 3: Left arm junction PCR using linear AC constructs capped with telomeric DNA at left arm only. Lane 4: Right arm junction PCR using linear AC constructs capped with telomeric DNA at left arm only. Lane 5: Left arm junction PCR using linear AC constructs capped with telomeric DNA at right arm only. Lane 6: Right arm junction PCR using linear AC constructs capped with telomeric DNA at right arm only. Lane 7: Left arm junction PCR using circular pLL-EHC DNA molecules. Lane 8: Right arm junction PCR using circular pLL-EHC DNA molecules.

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