Figure 2From: De novo assembly of potential linear artificial chromosome constructs capped with expansive telomeric repeatsSchematic diagram for generation of a linear AC construct. Two seed telomeric DNA vectors pLL-TBS and pLL-TSB were used to generate expanded back-to-back telomeric DNA fragments. A subsequent in vitro recombination reaction between the pLL-EHC vector and the back-to-back telomeric DNA resulted in a linear AC construct.Back to article page