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Table 1 Nicotiana benthamiana leaf fatty acid profiles

From: Rapid expression of transgenes driven by seed-specific constructs in leaf tissue: DHA production

Fatty acid    Construct pJP3057
Usual FA P19 only 35S DHA mix - 35S:LEC2 + 35S:LEC2
16:0 15.9 ± 0.2 16.6 ± 0.1 13.3 ± 0.1 13.2 ± 0.6
16:1Δ3t 1.7 ± 0.1 1.5 ± 0.1 1.3 ± 0.1 1.1 ± 0
16:3Δ9,12,15 6.3 ± 0.3 5.6 ± 0.1 7.1 ± 0.3 7.5 ± 0.4
18:0 3.6 ± 0.3 3.3 ± 0.1 1.8 ± 0.1 2.4 ± 0.3
18:1Δ9 2.8 ± 0.1 2.8 ± 0.2 1.1 ± 0.1 1.5 ± 0.2
18:2Δ9,12 18.7 ± 0.1 13.0 ± 0.1 13.8 ± 0.1 12.7 ± 0.4
18:3Δ9,12,15 45.6 ± 1.4 40.2 ± 0.5 56.3 ± 0.7 44.8 ± 2.1
20:0 1.3 ± 0.4 0.6 ± 0 0.3 ± 0 0.5 ± 0.1
Other minor 4.1 6.6 3.9 4.8
Total 100 90.4 98.9 88.5
New ω6 PUFA     
18:3Δ6,9,12 - 2.1 ± 0.2 - 2.4 ± 0.1
20:3Δ8,11,14 - - 0.2 ± 0.1 0.2 ± 0.1
20:4Δ5,8,11,14 - 0.2 ± 0 - -
Total 0 2.3 0.2 2.6
New ω3 PUFA     
18:4Δ6,9,12,15 - 2.0 ± 0
(16% Δ6-des)
0.9 ± 0.1 1.2 ± 0.1 (15% Δ6-des)
20:4Δ8,11,14,17 - 0.4 ± 0
(75% Δ6-elo)
- 2.0 ± 0.1
(85% Δ6-elo)
20:5Δ 5,8,11,14,17 - 0.3 ± 0
(93% Δ5-des)
- 0.6 ± 0
(71% Δ5-des)
22:5Δ7,10,13,16,19 - 2.3 ± 0
(94% Δ5-elo)
- 1.7 ± 0.1
(88% Δ5-elo)
22:6Δ 4,7,10,13,16,19 - 2.5 ± 0.1
(52% Δ4-des)
- 2.5 ± 0.2
(60% Δ4-des)
Total 0 7.5 0.9 11.1
Total new FA 0 14.0 1.1 13.0
Total FA 100 100 100 100
  1. Profile of fatty acid methyl esters produced directly from N. benthamiana leaf tissue transiently expressing single-gene CaMV 35S binary constructs and the seed-specific construct pJP3057. All infiltrations were performed with the P19 gene silencing suppressor and errors denote standard deviation of separate infiltrations performed in triplicate. Where shown, conversion efficiencies are calculated as p/(s + p) where p and s are the sum of all downstream products or substrates, respectively, and where both p and s are expressed as molar percentage of total fatty acids.