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Table 1 Nicotiana benthamiana leaf fatty acid profiles

From: Rapid expression of transgenes driven by seed-specific constructs in leaf tissue: DHA production

Fatty acid

  

Construct pJP3057

Usual FA

P19 only

35S DHA mix

- 35S:LEC2

+ 35S:LEC2

16:0

15.9 ± 0.2

16.6 ± 0.1

13.3 ± 0.1

13.2 ± 0.6

16:1Δ3t

1.7 ± 0.1

1.5 ± 0.1

1.3 ± 0.1

1.1 ± 0

16:3Δ9,12,15

6.3 ± 0.3

5.6 ± 0.1

7.1 ± 0.3

7.5 ± 0.4

18:0

3.6 ± 0.3

3.3 ± 0.1

1.8 ± 0.1

2.4 ± 0.3

18:1Δ9

2.8 ± 0.1

2.8 ± 0.2

1.1 ± 0.1

1.5 ± 0.2

18:2Δ9,12

18.7 ± 0.1

13.0 ± 0.1

13.8 ± 0.1

12.7 ± 0.4

18:3Δ9,12,15

45.6 ± 1.4

40.2 ± 0.5

56.3 ± 0.7

44.8 ± 2.1

20:0

1.3 ± 0.4

0.6 ± 0

0.3 ± 0

0.5 ± 0.1

Other minor

4.1

6.6

3.9

4.8

Total

100

90.4

98.9

88.5

New ω6 PUFA

    

18:3Δ6,9,12

-

2.1 ± 0.2

-

2.4 ± 0.1

20:3Δ8,11,14

-

-

0.2 ± 0.1

0.2 ± 0.1

20:4Δ5,8,11,14

-

0.2 ± 0

-

-

Total

0

2.3

0.2

2.6

New ω3 PUFA

    

18:4Δ6,9,12,15

-

2.0 ± 0

(16% Δ6-des)

0.9 ± 0.1

1.2 ± 0.1 (15% Δ6-des)

20:4Δ8,11,14,17

-

0.4 ± 0

(75% Δ6-elo)

-

2.0 ± 0.1

(85% Δ6-elo)

20:5Δ 5,8,11,14,17

-

0.3 ± 0

(93% Δ5-des)

-

0.6 ± 0

(71% Δ5-des)

22:5Δ7,10,13,16,19

-

2.3 ± 0

(94% Δ5-elo)

-

1.7 ± 0.1

(88% Δ5-elo)

22:6Δ 4,7,10,13,16,19

-

2.5 ± 0.1

(52% Δ4-des)

-

2.5 ± 0.2

(60% Δ4-des)

Total

0

7.5

0.9

11.1

Total new FA

0

14.0

1.1

13.0

Total FA

100

100

100

100

  1. Profile of fatty acid methyl esters produced directly from N. benthamiana leaf tissue transiently expressing single-gene CaMV 35S binary constructs and the seed-specific construct pJP3057. All infiltrations were performed with the P19 gene silencing suppressor and errors denote standard deviation of separate infiltrations performed in triplicate. Where shown, conversion efficiencies are calculated as p/(s + p) where p and s are the sum of all downstream products or substrates, respectively, and where both p and s are expressed as molar percentage of total fatty acids.
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Plant Methods

ISSN: 1746-4811