Figure 1From: Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell deathNucleic acid fragmentation in immature Scots pine seed. Nucleic acid fragmentation in the embryo surrounding region (ESR), cells of the megagametophyte tissue and the degenerated suspensor tissue in a developing Scots pine seed. (A) In the acridine orange (AO) -stained section, cell wall remnants and degraded nucleic acid formed a zone between the ESR and the developing embryo (arrows). (B) TUNEL-positive nuclei of the megagametophyte cells in the ESR (arrows). (C) TUNEL-positive signal in fragmented DNA among the cell wall remnants in the corrosion cavity (arrow). (D) AO-stained section with subordinate embryos and fragmented nucleic acids in the corrosion cavity close to the remnants of the degenerated suspensor tissue (arrows). CC = corrosion cavity, E = embryo, M = megagametophyte.Back to article page