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Table 2 Extraction efficiency of repeated extractions.

From: Proposal for field sampling of plants and processing in the lab for environmental metabolic fingerprinting

  Peaks [%] at t R = 0 - 14 min (mean ± sd) Peaks [%] at t R = 14 - 30 min (mean ± sd) Peaks [%] at t R = 30 - 38 min (mean ± sd) Peaks [%] at t R = 0 - 38 min (mean ± sd)
1. extraction 3.02 ± 0.59 77.64 ± 7.69 19.34 ± 8.17 41.61 ± 3.76
2. extraction 1.65 ± 0.46 53.47 ± 3.17 44.88 ± 3.20 64.91 ± 3.06
3. extraction 0 ± 0 34.68 ± 10.79 65.32 ± 10.79 72.04 ± 3.21
4. extraction 0.06 ± 2.86 28.82 ± 5.44 71.13 ± 5.50 87.75 ± 1.09
5. extraction 3.56 ± 2.48 31.50 ± 9.73 66.82 ± 12.38 94.93 ± 0.59
6. extraction 2.05 ± 1.13 30.68 ± 6.49 67.39 ± 4.86 98.07 ± 0.17
7. extraction 1.79 ± 0.68 29.54 ± 4.98 68.66 ± 3.59 100 ± 0
  1. Percentage (mean ± standard deviation) of metabolites (peaks) detected in 7 subsequent extractions of Plantago lanceolata leaf material, extracted in 2:1 methanol:dichloromethane and analysed by HPLC. Number of replicates N = 5. Columns 1-3 show the percentages of peaks eluting between different retention times (t R ) within one extraction step. Columns 1-3 add up horizontally to 100% for each extraction step. In the fourth column the sum of all 150 integrated peaks of all 7 extractions was set as 100%. This column indicates the cumulative values from one extraction to another.