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Table 2 Extraction efficiency of repeated extractions.

From: Proposal for field sampling of plants and processing in the lab for environmental metabolic fingerprinting

 

Peaks [%] at t R = 0 - 14 min (mean ± sd)

Peaks [%] at t R = 14 - 30 min (mean ± sd)

Peaks [%] at t R = 30 - 38 min (mean ± sd)

Peaks [%] at t R = 0 - 38 min (mean ± sd)

1. extraction

3.02 ± 0.59

77.64 ± 7.69

19.34 ± 8.17

41.61 ± 3.76

2. extraction

1.65 ± 0.46

53.47 ± 3.17

44.88 ± 3.20

64.91 ± 3.06

3. extraction

0 ± 0

34.68 ± 10.79

65.32 ± 10.79

72.04 ± 3.21

4. extraction

0.06 ± 2.86

28.82 ± 5.44

71.13 ± 5.50

87.75 ± 1.09

5. extraction

3.56 ± 2.48

31.50 ± 9.73

66.82 ± 12.38

94.93 ± 0.59

6. extraction

2.05 ± 1.13

30.68 ± 6.49

67.39 ± 4.86

98.07 ± 0.17

7. extraction

1.79 ± 0.68

29.54 ± 4.98

68.66 ± 3.59

100 ± 0

  1. Percentage (mean ± standard deviation) of metabolites (peaks) detected in 7 subsequent extractions of Plantago lanceolata leaf material, extracted in 2:1 methanol:dichloromethane and analysed by HPLC. Number of replicates N = 5. Columns 1-3 show the percentages of peaks eluting between different retention times (t R ) within one extraction step. Columns 1-3 add up horizontally to 100% for each extraction step. In the fourth column the sum of all 150 integrated peaks of all 7 extractions was set as 100%. This column indicates the cumulative values from one extraction to another.
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Plant Methods

ISSN: 1746-4811