|
Peaks [%] at t
R
= 0 - 14 min (mean ± sd)
|
Peaks [%] at t
R
= 14 - 30 min (mean ± sd)
|
Peaks [%] at t
R
= 30 - 38 min (mean ± sd)
|
Peaks [%] at t
R
= 0 - 38 min (mean ± sd)
|
---|
1. extraction
|
3.02 ± 0.59
|
77.64 ± 7.69
|
19.34 ± 8.17
|
41.61 ± 3.76
|
2. extraction
|
1.65 ± 0.46
|
53.47 ± 3.17
|
44.88 ± 3.20
|
64.91 ± 3.06
|
3. extraction
|
0 ± 0
|
34.68 ± 10.79
|
65.32 ± 10.79
|
72.04 ± 3.21
|
4. extraction
|
0.06 ± 2.86
|
28.82 ± 5.44
|
71.13 ± 5.50
|
87.75 ± 1.09
|
5. extraction
|
3.56 ± 2.48
|
31.50 ± 9.73
|
66.82 ± 12.38
|
94.93 ± 0.59
|
6. extraction
|
2.05 ± 1.13
|
30.68 ± 6.49
|
67.39 ± 4.86
|
98.07 ± 0.17
|
7. extraction
|
1.79 ± 0.68
|
29.54 ± 4.98
|
68.66 ± 3.59
|
100 ± 0
|
- Percentage (mean ± standard deviation) of metabolites (peaks) detected in 7 subsequent extractions of Plantago lanceolata leaf material, extracted in 2:1 methanol:dichloromethane and analysed by HPLC. Number of replicates N = 5. Columns 1-3 show the percentages of peaks eluting between different retention times (t
R
) within one extraction step. Columns 1-3 add up horizontally to 100% for each extraction step. In the fourth column the sum of all 150 integrated peaks of all 7 extractions was set as 100%. This column indicates the cumulative values from one extraction to another.