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Table 1 Differences in PCA scores between differently pre-treated samples.

From: Proposal for field sampling of plants and processing in the lab for environmental metabolic fingerprinting

Pre-treatment Scores of PC 1 (mean ± sd)   Scores of PC 2 (mean ± sd)  
Fresh leaves -1.68 ± 1.06 A 2.21 ± 1.01 a
N2 -3.24 ± 4.41 B 0.94 ± 1.00 ab
Lyophilisation -0.23 ± 1.89 AB -0.69 ± 1.53 ab
Air-dried -2.21 ± 0.54 AB -4.09 ± 4.75 b
  1. Loadings (mean ± standard deviation) of PC 1 and PC 2 of a PCA on metabolic fingerprints of the different pre-treatment methods (see Figure 1). Plantago lanceolata leaf material was either extracted directly, frozen in liquid nitrogen, lyophilised or air-dried for 24 hours at room temperature, before further extraction in 100% methanol. Extracts were analysed by HPLC. Number of replicates N = 5; air-dried samples N = 3. Differences among pre-treatments were tested with ANOVA followed by Tukey's HSD tests. Different upper case letters indicate significantly different scores of PC 1 (F3,14 = 3.121, P < 0.05); lower case letters indicate significantly different scores of PC 2 (F3,14 = 4.238, P < 0.01).