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Figure 3 | Plant Methods

Figure 3

From: NEATTILL: A simplified procedure for nucleic acid extraction from arrayed tissue for TILLING and other high-throughput reverse genetic applications

Figure 3

Evaluation of DNA quality and stability during long-term storage at 4°C. A- Integrity of DNA isolated in 96 deepwell plate after EtBr staining on agarose gel; B- Long term stability (Top panel) and PCR amplification (bottom panel) of nucleic acids. The DNA afer isolation (labeled 1-7) was suspended in either TE/RNase (Lane 1-6) or water (Lane 7) and stored at 4°C. At different time periods (0, 4, 9 and 15 months) from isolation DNA was checked for integrity using agarose gel electrophoresis. The quality of DNA was checked by PCR amplification of actin gene (primers as in Fig. 2) using aliquots from stored DNA at 3, 6 and 15 months. M-Markers, Mo-Months.

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