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Figure 2 | Plant Methods

Figure 2

From: Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells

Figure 2

qPCR analysis of RBC-L and RBC-S transcripts in A. thaliana and evaluation of reference genes. (A) Transcript levels of RBC-L and RBC-S in A. thaliana rosette leaf samples were analyzed by qRT-PCR and quantified by the ΔΔCt method using the reference genes indicated at bottom. Data reflect relative transcript accumulation in abc4 plants relative to that in wild-type plants. (B) Transcript levels of the reference genes in rosette leaf samples from A. thaliana were analyzed by qRT-PCR and quantified by the ΔΔCt method using genomic DNA as the reference. Data reflect relative transcript accumulation in abc4 plants relative to that in wild-type plants. (C) Transcripts numbers per cell in the wild-type A. thaliana rosette leaf were determined relative to genomic DNA using qPCR analysis. Data reflect the mean ± s.d. from duplicate experiments of four biological samples.

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Plant Methods

ISSN: 1746-4811