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Figure 2 | Plant Methods

Figure 2

From: Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum

Figure 2

Mycorrhiza colonization and nodule numbers in roots of Pisum sativum inoculated with pCAPE2-PsSym19. (A) Percentage of total root length with arbuscules 10 and 20 days post transfer (dpt) and vesicles 20 dpt in plants agro-inoculated with pCAPE2-Con (control, white columns) or pCAPE2-PsSym19 (Sym19, grey columns). Results are presented for one of two experiments showing similar results. (B) Number of visible nodules per plant 20 dpt. Plants were agro-inoculated with pCAPE2-Con (control, white columns) or pCAPE2-PsSym19 (Sym19, grey columns). Plants were grown in the mycorrhiza-PEBV-VIGS set-up "Myc Exp" (described in this paper) or in the nodulation -VIGS setup "Nod Exp" [5]. Columns and bars are means ± 95% confidence intervals (CI). VIGS effect was significant for all measured variables.

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