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Figure 1 | Plant Methods

Figure 1

From: Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum

Figure 1

Protocol for virus-induced gene silencing for functional studies of mycorrhiza-related genes of Pisum sativum. Day 1: Sow pre-germinated cucumber seeds (right pot) in nylon mesh bag in the "nurse pots". Day 14: Sow pre-germinated pea seeds (left pot) in the "pea pot". Day 25: Agro-inoculate pea plants with pea early-browning virus VIGS constructs. Day 37-39: Transfer pea pots to nurse pots. *Day 41-43: In experiments involving nodulation analysis, add R. leguminosarum to nurse pot with in-growing pea roots. Day 46-59: Harvest and wash pea roots growing into the nurse pots. Analyze for mycorrhiza colonization, gene expression and nodulation.

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Plant Methods

ISSN: 1746-4811