Figure 4
From: pH-sensitivity of YFP provides an intracellular indicator of programmed cell death
Loss of YFP fluorescence during xylem development. (A) Five day old Arabidopsis root expressing both YFP::CesA7 and mCherryER under the xylem specific promoter of the cellulose synthase subunit IRX3. White bars indicate two adjacent xylem cells (1 & 2). Left panel shows YFP::CesA7 fluorescence. Centre panel shows mCherryER fluorescence. Right panel shows both the YFP-CesA7 and mCherry ER fluorescence merged with the bright field image. * highlights the typical banding pattern characteristic of xylem. Images were captured using a YFP/mCherry dual (51019) filter. (B) Time-lapse photography of two adjacent xylem cells (1 & 2) in the same line as above. White dotted lines highlight the cell wall between cells 1 & 2. Green: YFP fluorescence; red: mCherry fluorescence.