Loss of YFP fluorescence induced by BAX can be prevented by AtBAXI-1 and Bcl-2. (A) A typical construct for the gene of interest fused to YFP, the right panel shows an YFP expressing cell. (B) Various constructs for the reference gene. The right panel depicts a typical RFP td-tomato expressing cell. (C) Onion cells transfected using p35S::YFP, p35S::BAX::YFP (BAX), p35S::BAX::YFP (BAX) + p35S::Bcl-2 or p35S::BAX::YFP (BAX) + p35S::AtBI-1 (AtBAXI-1). p35S::Bcl-2 or p35S::AtBI-1 plasmids are co-transfected in a two-fold molar excess compared to p35S::BAX::YFP. p35S::GUS was co-transfected as the transfection reference. YFP positive cells are scored and expressed as a % of GUS expressing cells. Errors bars represent 2× SE of triplicates of 100 cells each.