Figure 2

Silencing of HvIPS1 in barley roots and shoots. A HvIPS1 RNA expression levels in root tissue determined by qRT-PCR (normalization to ubiquitin). Plants inoculated with BSMV-IPS1 (black bars) or BSMV-GFP²⁵⁰ (white bars). Plants were grown in 0 (5, 7, 9 dpi) or 1 mM Pi (9 dpi) and harvested at 5, 7, or 9 dpi. Each bar represents three samples (with one exception - only two samples for the 0 Pi, GFP, 9dpi bar). Error bars denote standard deviations. AU - Arbitrary units. B Stability of the BSMV-IPS1 construct in roots of inoculated plants. cDNA prepared for qRT-PCR (A) was used for PCR with primers flanking the insert. Lanes 1, 2, 3: 0 mM Pi, 5 dpi; 4, 5, 6: 0 mM Pi, 7 dpi; 7, 8, 9: 0 mM Pi, 9 dpi; 10, 11, 12: 1 mM Pi, 9 dpi; 13: plasmid containing BSMVγ-IPS1; 14: water control. M4: GeneRuler 50 bp DNA Ladder (Fermentas); white arrow represents DNA fragment of 500 bp; bands below are 400, 300, and 250 bp. C Stability of the BSMV-GFP²⁵⁰ construct in roots of inoculated plants. cDNA prepared for qRT-PCR (panel A) was used for PCR with primers flanking the insert. Lanes 1, 2, 3: 0 mM Pi, 5 dpi; 4, 5, 6: 0 mM Pi, 7 dpi; 7, 8: 0 mM Pi, 9 dpi; 9, 10, 11: 1 mM Pi, 9 dpi. D HvIPS1 RNA expression levels in root and shoot tissue determined by qRT-PCR (normalization to ubiquitin). BSMV-IPS1 (black bars), BSMV-GFP²⁵⁰ (white bars). Plant were grown in 0 mM Pi and harvested at 9 dpi. Each bar represents five samples. Error bars denote standard deviations.