Figure 4
Employing the DPI-ELISA to other transcription factors. A. The DNA-sequences of the double stranded GA-probe and the mutated oligonucleotide version (GAm-probe) are given in 5' to 3'-orientation for the sense strand; mutated bases are highlighted (red) [25]. The specific binding of At BPC2 to the GABio-probe was shown by a competition experiment with non-biotinylated dsDNA (B.). Different amounts of GA- or GAm-probes (0, 100, 1000 pmol) were added to At BCP2 crude extract and incubated on an ELISA-plate coated with 2 pmol of biotinylated GABio-probe. The biotinylated double stranded GAmBio-probe incubated with At BPC2 extract served as negative control. Representative wells of the microtiter plate are shown below the graph for visual inspection. The grey background indicates the negative control (untransformed BL21/RIL cells) reference values in percent.