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Figure 7 | Plant Methods

Figure 7

From: A simple, rapid method to isolate salt glands for three-dimensional visualization, fluorescence imaging and cytological studies

Figure 7

Comparison of microtome salt gland section with isolated salt gland of A. officinalis. (A) Microtome section of pre-fixed paraffin-embedded leaf specimen showing cuticle envelope (cu) surrounding the salt gland and above the epidermal cells (epc). Bar = 20 μm. (B) Three-dimensional image of isolated salt gland (side view) showing 4 secretory cells (sc) above the stalk cell (st). The three-dimensional image was constructed using Imaris™ (beta) 3.0, based on stacked images captured with Zeiss LSM 510. Excitation and emission wavelengths were 488 nm (43%) and >505 nm, respectively. Bar = 10 μm. Note the presence of two collecting cells (cc) in (A) which are lacking in the isolated salt gland (B).

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Plant Methods

ISSN: 1746-4811