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Figure 5 | Plant Methods

Figure 5

From: A simple, rapid method to isolate salt glands for three-dimensional visualization, fluorescence imaging and cytological studies

Figure 5

Optical sections of an isolated salt gland of A . officinalis. Sections (A-E) were taken in the fluorescent mode to show the side views of a salt gland. Details within cells could be observed due to the green autofluorescent nature of the salt gland. Note the presence of strongly autofluorescent structures (arrows) in the middle or periphery of some secretory cells. (F) Corresponding bright-field image of (C) taken together with overlapping image recorded simultaneously in the transmission and fluorescent modes. Optical sections were taken with Zeiss LSM 510. Excitation and emission wavelengths were 488 nm (10%) and >530 nm, respectively. Bars = 5 μm.

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Plant Methods

ISSN: 1746-4811