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Figure 1 | Plant Methods

Figure 1

From: The Rg1 allele as a valuable tool for genetic transformation of the tomato 'Micro-Tom' model system

Figure 1

Introgression of the Rg1 allele into the Micro-Tom (MT) background. The cultivar MsK harbors Rg1 linked to the r (yellow flesh) allele in chromosome 3, which confers yellow color to fruits and was used as a morphological marker. MT was used as pollen receptor in all crosses. In F2, recombinants with MT small size and yellow fruits (rr) phenotype were selected and allowed to self-pollinate for six generations (F6) rendering to the true-type Micro-MsK [17], which was used for further backcrosses (BC) with MT. After every two backcrosses (BC), plants were allowed to self-pollinate rendering to BC2F2, BC4F2 and BC6F2, which were used to select Rg1 based on the yellow fruit trait. Seeds from rr BC6F2 plants (BC6F3 seeds) were germinated in vitro in order to confirm their high shoot formation phenotype from root or cotyledon explants, indicating the presence of Rg1. After six generations of self-pollination (BC6F6) the true-type genotype harboring the morphological marker r and the high regeneration allele Rg1 was named MT-Rg1

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