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Figure 4 | Plant Methods

Figure 4

From: Isolation of intact sub-dermal secretory cavities from Eucalyptus

Figure 4

A non-volatile component remains within the lumena of Eucalyptus secretory cavities after steam distillation of volatile essential oils. (a) Steam-distilled Eucalyptus polybractea leaf with a portion of epidermis and attached cuticle dissected away and mesophyll cells brushed off to expose the secretory cavities. Non-volatile material remains within cavity lumena as translucent-brown material (arrow). (b) Secretory cavities of E. globulus (top cavity) and E. polybractea (bottom cavity) dissected from steam-distilled leaves and brushed free of mesophyll cells to highlight the highly abundant non-volatile material (arrow). (c) Non-volatile material extracted from cavities of steam-distilled leaves with a microprobe. All scale bars represent 100 μm.

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