Figure 2
From: Isolation of intact sub-dermal secretory cavities from Eucalyptus
Application of microscopy techniques to essential oil secretory cavities isolated from Eucalyptus polybractea leaves. (a) Isolated cavity viewed under transmitted light, the arrow indicates the estimated extent of the lumen and the approximate position of microprobe insertion in panel c. (b) Isolated cavity viewed with UV excitation (UV filter) showing green autofluorescence of the non-volatile resinous material. (c) Punctured cavity stained with nile red and viewed under UV excitation (UV filter), remaining essential oils fluoresce red, but the resinous component (green autofluorescence) remains unstained. (d) Confocal micrograph of an isolated cavity showing outer cell arrangement. (e) Scanning electron micrograph detailing outer cavity cells. (f) Isolated cavity viewed under UV excitation (UV filter) after Hoechst vital staining showing the integrity of cavity cell nuclei (bright blue fluorescence) and the green autofluorescence of the resinous component within the lumen. All scale bars represent 50 μm.