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Figure 6 | Plant Methods

Figure 6

From: High frequency, cell type-specific visualization of fluorescent-tagged genomic sites in interphase and mitotic cells of living Arabidopsis plants

Figure 6

Visualization of fluorescent tagged sites on mitotic chromosomes. In the division zone of the root, chromosomes are dual labelled with H2B-mRed and EGFP-LacI (both genes are under control of the RPS5 promoter). (A) Mitotic chromosomes at the metaphase plate are viewed as bright red bars (white arrow; shown enlarged in B) with EGFP-tagged sites visible as two clustered pairs of green fluorescent dots (C; merged image in D). (E) Mitotic chromosomes at anaphase with two pairs of EGFP-tagged sites (F; merged image in G). Interphase nuclei are dull red ovals (A, E, G). In the merged image (G), three neighbouring interphase nuclei with superimposed green fluorescent dots can be seen. All bars indicate 5 μm. Homozygous tagged line 112 was used for A-D; homozygous tagged line 26 was used for E-G. In both cases, the EGFP-LacI fusion protein was supplied in trans.

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Plant Methods

ISSN: 1746-4811