Table 2 PCR primer sequences and tags used for the amplification and sequencing of the BrWD40 genomic region.
From: Targeted identification of genomic regions using TAGdb
Primer pair | Primer sequence (5'-3') | Tag name | Primer position relative to AtWD40 start codon (bp) | B. rapa product size (bp) |
|---|---|---|---|---|
1F | TCGGACAAGGAGACGGGTCC | BrC_27_001_56-123-1844_2 | 290 | a: 2862 |
1R | AAGAGCTGGTACACAGGTAACG | BrC_27_001_56-123-1844_1 | 3224 | Â |
2F | CAAAAATAACTCACATTGACATCAA | BrC_37_001_40-284-1268_1 | (no match) | b: 2945 |
2R | TCAGAACAGGAAGGTCAATTTC | BrC_37_001_40-284-1268_2 | 2070 | Â |
Sequencing primers | ||||
3F | GGTGCTGGATTCATGCTTTAC | BrC_03_002_4-32-317-1572-1 | 1123 | Â |
3R | CTGATTGCAGGAGGACACAA | BrC_03_002_4-32-317-1572-2 | (no match) | Â |
4F | GGGCATGCCATCTGTTATATG | BrC_03_001_3-13-638-284-2 | (no match) | Â |