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Figure 1 | Plant Methods

Figure 1

From: High-contrast three-dimensional imaging of the Arabidopsis leaf enables the analysis of cell dimensions in the epidermis and mesophyll

Figure 1

Leaf image problems and image quality difference between confocal and multiphoton systems. (a-c) Problems encountered upon three-dimensional imaging of leaves include: (a) starch granules (some indicated by arrows), (b) low contrast between cell walls (cw) and intracellular (ics) and intercellular spaces (bcs), and (c) wrinkling (some of it indicated by arrows) in Arabidopsis mesophyll cells (scale bars of 25 μm). (d) Single sections of an image stack of an Arabidopsis leaf (leaf 6, 16 days after initiation) acquired by confocal (left) or multiphoton (right) microscopy (scale bar of 50 μm for all images): sections in the spongy mesophyll at 35 μm in depth (top) and the palisade mesophyll at 125 μm in depth (bottom).

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Plant Methods

ISSN: 1746-4811