Figure 4
From: UCE: A uracil excision (USER™)-based toolbox for transformation of cereals
A-D Stable transformation (Agrobacterium-mediated) - UBI:eGFP-ATG8 roots compared with wild type and UBI:eGFP. A, Dark-field. B, e GFP epifluorescence. C-D, CLSM of roots. C, UBI:eGFP D, UBI:eGFP-ATG8 (Arrow indicates cell with many autophagosomes), E-H Transient transformations (biolistic) - endosperm cells with transgenic eGFP or mCHERRY epifluorescence. E, D-Hord:eGFP F, D-Hord:TP-eGFP G, D-Hord:SP-mCHERRY.