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Figure 3 | Plant Methods

Figure 3

From: UCE: A uracil excision (USER™)-based toolbox for transformation of cereals

Figure 3

Transgene over-expression of HvHB1 in endosperm. Relative level of endogenous and transgenic expression of HvHB1 in the endosperm. A, The transgenic cDNA contains the 5' UTR of D-hordein. This was used to design a primer pair that recognizes only transgenic expression of HvHB1 (lower illustration) whereas a primer pair inside the coding region of HvHB1 recognizes both endogenous and transgenic expression of HvHB1 (upper illustration). B, Expression in the endosperm was determined by qRT-PCR in wild type barley and 20 putative transgenic lines transformed with pUCED-Hord:HvHB1:NOS. The expression level of endogenous HvHB1 in the wild type plants was defined to be 1.

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