Skip to main content
Figure 1 | Plant Methods

Figure 1

From: Rapid metabolic profiling of Nicotiana tabacum defence responses against Phytophthora nicotianae using direct infrared laser desorption ionization mass spectrometry and principal component analysis

Figure 1

Workflow of the combined IR-LDI mass spectrometry - principal component analysis of plant metabolites. Schematic workflow of the analysis: (I) sample treatment, (II) sample collection, (III) direct IR-LDI-oTOF MS measurement, (IV) principal component analysis. The lower cuticle of the leaf is removed using adhesive tape and disks are placed upside down on a glass slide. The glass slide is mounted in a custom-made sample plate holder and transferred into the laser mass spectrometer for measurement. The nomenclature employed to identify the sample type is explained in the text.

Back to article page