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Figure 2 | Plant Methods

Figure 2

From: Protocol: a simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species

Figure 2

An example of multi-sample SNP genotyping. To demonstrate multi-sample SNP genotyping, we mapped the lrt5 region using an F2 population. (A) Scatter plots showing genotyping results for the S1919 (left) and S0575 (right) markers. The X- and Y-axis represent fluorescence intensity value of 'Koshihikari' and 'Kasalath' allele-specific amplification, respectively. (B) The fluorescence data of 'Koshihikari' (upper tier in each field) and 'Kasalath' (lower tier) that correspond to the data in (A) are shown in the upper two panels in the 96-well format. Red, blue and yellow indicate 'Koshihikari' homozygotes, 'Kasalath' homozygotes, and heterozygotes of the allele, respectively. In the lower panel, recombination events between the markers S1919 and S0575 are indicated in red. Recombination was determined from the genotypes indicated in the upper two panels. (C) Genetic map of the lrt5 region, derived from the genotypes of 17 recombinants as analyzed using three additional ASP-PCR markers. White and black bars represents 'Koshihikari' and 'Kasalath' alleles, respectively. Number of recombinants are given in parenthesis.

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