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Table 2 DNA yield or recovery rate during silica-mediated purification in this study

From: Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology

Material DNA Sub-protocol Input DNA productiona
     Yield OD260/280
E. coli 35S-WRKY29g-GFP-MYC 1 2 ml culture OD600 = 2 6.2 ± 0.3 μg 1.91 ± 0.03
E. coli pCB302 1 2 ml culture OD600 = 2 2.6 ± 0.4 μg 1.93 ± 0.03
E. coli pBI101 1 2 ml culture OD600 = 2 2.4 ± 0.3 μg 1.92 ± 0.02
E. coli pPZP222 1 2 ml culture OD600 = 2 4.7 ± 0.4 μg 1.88 ± 0.02
A. tumefaciens VKH-NLS-YFP-GUS 1 2 ml culture OD600 = 2 0.4 ± 0.03 μg 1.95 ± 0.03
DNA solution AtWRKY29 gPCR 2 1 μg DNA 78 ± 6%b 1.81 ± 0.05
Agarose gel AtWRKY29 gPCR 2 0.5 μg DNA 68 ± 2%b 1.85 ± 0.02
Arabidopsis (s)c genomic DNA 3 10 mg tissue 1.7 ± 0.1 μg 1.90 ± 0.02
Arabidopsis (l)d genomic DNA 3 10 mg tissue 1.1 ± 0.1 μg 1.93 ± 0.02
tobacco (s)e genomic DNA 3 10 mg tissue 1.9 ± 0.2 μg 1.93 ± 0.01
maize (l)f genomic DNA 3 10 mg tissue 1.0 ± 0.1 μg 1.88 ± 0.03
  1. a. Qualities and quantities of DNA products were measured by NanoDrop ND-1000 Spectrophotometer (Thermo Scientific) and each DNA purification was repeated for at least 5 times.
  2. b. DNA recovery rate (i.e., yield/input × 100%) is shown instead of DNA yield.
  3. c. Severn-day-old Arabidopsis seedlings (s) were used
  4. d. Four-week-old Arabidopsis leaves (l) were used
  5. e. Seven-day-old tobacco seedlings (s) were used
  6. f. Ten-day-old maize leaves (l) were used