Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology

Table 2 DNA yield or recovery rate during silica-mediated purification in this study

Material	DNA	Sub-protocol	Input	DNA production^a
				Yield	OD260/280
E. coli	35S-WRKY29g-GFP-MYC	1	2 ml culture OD600 = 2	6.2 ± 0.3 μg	1.91 ± 0.03
E. coli	pCB302	1	2 ml culture OD600 = 2	2.6 ± 0.4 μg	1.93 ± 0.03
E. coli	pBI101	1	2 ml culture OD600 = 2	2.4 ± 0.3 μg	1.92 ± 0.02
E. coli	pPZP222	1	2 ml culture OD600 = 2	4.7 ± 0.4 μg	1.88 ± 0.02
A. tumefaciens	VKH-NLS-YFP-GUS	1	2 ml culture OD600 = 2	0.4 ± 0.03 μg	1.95 ± 0.03
DNA solution	AtWRKY29 gPCR	2	1 μg DNA	78 ± 6%^b	1.81 ± 0.05
Agarose gel	AtWRKY29 gPCR	2	0.5 μg DNA	68 ± 2%^b	1.85 ± 0.02
Arabidopsis (s)^c	genomic DNA	3	10 mg tissue	1.7 ± 0.1 μg	1.90 ± 0.02
Arabidopsis (l)^d	genomic DNA	3	10 mg tissue	1.1 ± 0.1 μg	1.93 ± 0.02
tobacco (s)^e	genomic DNA	3	10 mg tissue	1.9 ± 0.2 μg	1.93 ± 0.01
maize (l)^f	genomic DNA	3	10 mg tissue	1.0 ± 0.1 μg	1.88 ± 0.03

a. Qualities and quantities of DNA products were measured by NanoDrop ND-1000 Spectrophotometer (Thermo Scientific) and each DNA purification was repeated for at least 5 times.
b. DNA recovery rate (i.e., yield/input × 100%) is shown instead of DNA yield.
c. Severn-day-old Arabidopsis seedlings (s) were used
d. Four-week-old Arabidopsis leaves (l) were used
e. Seven-day-old tobacco seedlings (s) were used
f. Ten-day-old maize leaves (l) were used

ISSN: 1746-4811