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Table 1 Seven solutions used in the silica protocol

From: Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology

Solution Name Solution Composition Storage Usage
Solution A
(Resuspension solution)
50 mM Tris-HCl, pH 7.5, 10 mM EDTA, 100 g/ml RNase A 4°C Plasmid miniprep, genomic DNA extraction
Solution B
(Alkaline lysis solution)
0.2 M NaOH, 1% sodium dodecyl sulfate (SDS) Room temp. Plasmid miniprep
Solution C
(Neutralization solution)
1.32 M KOAc, pH 4.8 Room temp. Plasmid miniprep
Solution D
(NaI solution)
6 M NaI 4°C Plasmid miniprep, PCR/Gel purification, genomic DNA extraction
Solution E
(Washing solution)
50% ethanol, 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA Room temp. Plasmid miniprep, PCR/Gel purification, genomic DNA extraction
Solution F 10% SDS Room temp. Genomic DNA extraction
Solution G Phenol:chloroform:isoamyl alcohol (25:24:1, v/v) 4°C Genomic DNA extraction
  1. Solution D is stored in darkness; Solution G is covered by water phase with β-mercaptoethanol