Table 1 Seven solutions used in the silica protocol

Solution A

(Resuspension solution)

50 mM Tris-HCl, pH 7.5, 10 mM EDTA, 100 g/ml RNase A

4°C

Plasmid miniprep, genomic DNA extraction

Solution B

(Alkaline lysis solution)

0.2 M NaOH, 1% sodium dodecyl sulfate (SDS)

Room temp.

Plasmid miniprep

Solution C

(Neutralization solution)

1.32 M KOAc, pH 4.8

Room temp.

Plasmid miniprep

Solution D

(NaI solution)

6 M NaI

4°C

Plasmid miniprep, PCR/Gel purification, genomic DNA extraction

Solution E

(Washing solution)

50% ethanol, 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA

Room temp.

Plasmid miniprep, PCR/Gel purification, genomic DNA extraction

Solution F

10% SDS

Room temp.

Genomic DNA extraction

Solution G

Phenol:chloroform:isoamyl alcohol (25:24:1, v/v)

4°C

Genomic DNA extraction