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Figure 3 | Plant Methods

Figure 3

From: Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology

Figure 3

Miniprep DNA by the silica protocol allows a long read length in DNA sequencing. The 3' end of each DNA sequencing result beyond 700 bp is shown with individual nucleotide peaks clearly distinguishable. The alignment of the sequencing results of 35S-WRKY29g-GFP plasmid (upper panel) and its derivative 35S-WRKY29g-GFP-MYC (lower panel) validated a successful insertion of a MYC-epitope tag coding sequence between the BglII/NotI sites in the 35S-WRKY29g-GFP plasmid. The MYC-tag (EQKLISEEDL) coding sequence is labeled by a line on top. The numbers underneath the DNA sequence were generated by the sequence reading software 4Peaks http://mekentosj.com/science/4peaks/ to indicate the read length. Note that the BglII site of the 35S-WRKY29g-GFP plasmid was removed after ligation to the BamHI site in front of the MYC-tag coding sequence.

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Plant Methods

ISSN: 1746-4811