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Table 5 Silencing of Magnesium chelatase (su): Quantification of Magnesium chelatase (su) mRNA in three pWSRiA:ChlI versus control treatment

From: Development of a gene silencing DNA vector derived from a broad host range geminivirus

Treatment Mean ct Mg Chelatase ± s.e. Mean ct 18S ± s.e. Delta ct ± 98% confidence limit Relative Mg chelatase mRNA (2Delta-Delta ct)
pWSRiA:su 26.96 ± 0.05 27.34 ± 0.08 -0.38 ± 0.10 0.21
pWSRiA:su 24.67 ± 0.06 25.72 ± 0.06 -1.06 ± 0.10 0.33
pWSRiA:su 24.39 ± 0.08 25.64 ± 0.05 -1.61 ± 0.11 0.48
pWSRiA:CAT 25.26 ± .010 27.92 ± 0.17 -2.66 ± 0.22 1.00
  1. Mean ct (threshold cycle) values were calculated from three replicates per cDNA sample. Delta ct values were calculated as the difference in ct values of Mg chelatase and 18S amplifications for each sample. Relative Mg chelatase mRNA values were calculated as 2 to the power of the delta ct minus delta ct of the control (delta-delta values).