Development of a gene silencing DNA vector derived from a broad host range geminivirus

Table 2 Silencing of rbcS and transketolase

Treatment^a	Plants silenced after treatment
a. Initial Trials
1. no DNA (mock)	0 of 6 plants
2. pWSRi	0 of 6 plants
3. pBCTV.R1	0 of 6 plants
4. pWSRi + pBCTV.R1	0 of 6 plants
5. pWSRi:rbcS.	6 of 6 plants
6. pWSRi:rbcS + pBCTV.R1	6 of 6 plants
7. pWSRi:transketolase	6 of 6 plants
8. pWSRi:transketolase + pBCTV.R1	6 of 6 plants
b. Secondary Trials
pWSRi:rbcS.	12 strong to moderate
	5 medium to light
	1 no response
pWSRi:transketolase	12 strong to moderate
	3 no response
	3 dead

^aTungsten particles were precipitated with the indicated DNAs and used to biolistically inoculate spinach plants. Plants were scored for leaf bleaching indicative of silencing beginning 6 weeks post-inoculation.

ISSN: 1746-4811