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Table 1 Recombinant binary plasmids used in this study

From: The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species

Binary plasmid

Host vector

Resistance

Promoter

ORF

Reference

pPZP-hRLuc

pPZP222

Spectinomycin

d35Sa

hRLuc

[23]

pVKH-NLS-YFP-GUS

pVKH18

Kanamycin

d35S

NLS-YFP-GUS

This study

px-cb

pFGC19

Kanamycin

d35S

Peroxisome-CFP

[25]

mt-yb

pFGC19

Kanamycin

d35S

Mitochondria-YFP

[25]

pFGC-YFP-1CCGT

pFGC19

Kanamycin

d35S

YFP-MYA1CCGT

[27]

pFGC-YFP-FABD2

pFGC19

Kanamycin

d35S

YFP-FABD2

This study

pVKH-YFP-At3g51660

pVKH18

Kanamycin

d35S

YFP-AT3g51660

This study

pVKH-At1g01170-YFP

pVKH18

Kanamycin

d35S

At1g01170-YFP

This study

pPZP-At2g47840-YFP

pPZP222

Spectinomycin

d35S

At2g47840-YFP

This study

pt-cb

pFGC19

Kanamycin

d35S

Plastid-CFP

[25]

ER-yk

pBIN20

Kanamycin

d35S

ER-YFP

[25]

ER-ck

pBIN20

Kanamycin

d35S

ER-CFP

[25]

G-yk

pBIN20

Kanamycin

d35S

Golgi-YFP

[25]

G-ck

pBIN20

Kanamycin

d35S

Golgi-CFP

[25]

mt-ck

pBIN20

Kanamycin

d35S

Mitochondria-CFP

[25]

px-ck

pBIN20

Kanamycin

d35S

Peroxisome-YFP

[25]

pt-yk

pBIN20

Kanamycin

d35S

Plastid-YFP

[25]

pVKH-YFP

pVKH18

Kanamycin

d35S

YFP

This study

pPZP-Cer

pPZP222

Spectinomycin

d35S

Cerulean

This study

pVKH-YFP-Cer

pVKH18

Kanamycin

d35S

YFP-Cerulean

This study

pBIN-YFP-HY5

pBIN20

Kanamycin

d35S

YFP-HY5

[23]

pPZP-Cer-HY5

pPZP222

Spectinomycin

d35S

Cerulean-HY5

This study

pVKH-YN-HY5

pVKH18

Kanamycin

d35S

YN-HY5

This study

pPZP-YC-HY5

pPZP222

Spectinomycin

d35S

YC-HY5

This study

pVKH-YFP-MYA1

pVKH18

Kanamycin

MYA1pro b

YFP-MYA1

This study

pVKH-GUS

pVKH18

Kanamycin

Ubi-1 c

GUS

This study

  1. a d35S, double cauliflower mosaic virus 35S promoter.
  2. b MYA1pro, MYA1 native promoter.
  3. c Ubi-1, maize ubiquitin 1 promoter.
  4. Note that 4 types of binary vectors with diverse genes have been tested.