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Figure 3 | Plant Methods

Figure 3

From: The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species

Figure 3

Subcellular localization studies by the FAST assays. A, YFP-At3g51660 (green) targeted to peroxisomes labeled by Peroxisome-CFP (Magenta) as indicated by the merged image. Arabidopsis seedlings were cocultivated simultaneously with agrobacteria cells carrying d35S::YFP-At3g51660 construct and those carrying d35S::Peroxisome-CFP marker. B, At1g01170-YFP (green) targeted to mitochondria labeled by Mitochondria-CFP (Magenta) as indicated by the merged image. Transgenic Arabidopsis seedlings expressing Mitochondria-CFP marker were cocultivated with agrobacteria cells carrying d35S::At1g01170-YFP construct. C, At2g47840-YFP (green) targeted to the envelope of chloroplasts labeled by autofluorescence (Magenta) as indicated by the merged image. Arabidopsis seedlings were cocultivated with agrobacteria cells carrying d35S::At2g47840-YFP construct. D, At2g47840-YFP (green) targeted to the envelope of plastids labeled by Plastid-CFP (Magenta) as indicated by the merged image. Arabidopsis seedlings were cocultivated with agrobacteria cells simultaneously carrying d35S::At2g47840-YFP and d35S::Plastid-CFP constructs. Scale bar = 15 μm.

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