Figure 2From: The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant speciesTransient expression of various constructs in Arabidopsis by the FAST assays. All cocultivations were carried out for 40 hr in the presence of 0.005% Silwet L-77 and bacteria of OD600 = 0.5. A, d35S::Peroxisome-CFP marker in wild-type seedling. Scale bar = 60 μm. B, d35S::YFP-FABD2 in wild-type seedling. Scale bar = 20 μm. C, d35S::YFP-MYA1CCGT in wild-type seedling. Scale bar = 60 μm. D, ubi-1::GUS in 33 wild-type seedling. Scale bar = 0.5 mm. E, MYA1pro::YFP-MYA1 in wild-type seedling. Scale bar = 60 μm. F, MYA1pro::YFP-MYA1 in mya1 mutant seedling. Scale bar = 60 μm. G, d35S::NLS-YFP-GUS in eif3h mutant seedling. Scale bar = 60 μm.Back to article page