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Figure 2 | Plant Methods

Figure 2

From: The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species

Figure 2

Transient expression of various constructs in Arabidopsis by the FAST assays. All cocultivations were carried out for 40 hr in the presence of 0.005% Silwet L-77 and bacteria of OD600 = 0.5. A, d35S::Peroxisome-CFP marker in wild-type seedling. Scale bar = 60 μm. B, d35S::YFP-FABD2 in wild-type seedling. Scale bar = 20 μm. C, d35S::YFP-MYA1CCGT in wild-type seedling. Scale bar = 60 μm. D, ubi-1::GUS in 33 wild-type seedling. Scale bar = 0.5 mm. E, MYA1pro::YFP-MYA1 in wild-type seedling. Scale bar = 60 μm. F, MYA1pro::YFP-MYA1 in mya1 mutant seedling. Scale bar = 60 μm. G, d35S::NLS-YFP-GUS in eif3h mutant seedling. Scale bar = 60 μm.

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