Skip to main content

Table 1 Method validation – linearity, reproducibility and recovery.

From: Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues

Compound

m/z §

RI #

Linearity, R 2

Reproducibility, % variance

Recovery, %

n-Dodecane (C12) (RT Standard 1)

85

1200

   

n-Pentadecane (C15) (RT Standard 2)

85

1500

   

n -Tetradecanoic acid (14:0) (TMS)

242

1850

0.992

10.2

nd

n-Nonadecane (C19) (RT Standard 3)

85

1900

   

n -Pentadecanoic acid (15:0) (TMS)

299

1947

0.977

10.1

nd

n -Hexadecanol (1OH-16:0) (TMS)

299

1959

0.989

10.4

105

n -Hexadecenoic acid (16:1) (TMS)

311

2021

0.994

19.6

nd

n -Hexadecanoic acid (16:0) (TMS)

313

2047

0.981

6.8

97

n -Heptadecanoic acid (17:0) (TMS)

327

2144

0.996

9.2

nd

n -Octadecanol (1OH-18:0) (TMS)

327

2154

0.991

7.1

102

n-Docosane (C22) (Rt Standard 4)

85

2200

   

n -9,12-Octadecadienoic acid (18:2) (TMS)

337

2210

0.925

17.4

nd

n -Octadecenoic acid (18:1) (TMS)

339

2215

0.999

13.2

75

n-Nonadecanoic acid methyl ester (IS)

269

2232

   

n -Octadecanoic acid (18:0) (TMS)

341

2243

0.977

7.9

103

n -Eicosanol (TMS) (1OH-20:0)

355

2362

0.998

5.9

91

n -Eicosenoic acid (20:1) (TMS)

367

2401

0.995

14.7

nd

n -Eicosanoic acid (20:0) (TMS)

369

2454

0.989

19.1

84

n -Docosanoic acid (22:0) (TMS)

397

2649

0.996

15.6

nd

n -Heptacosane (C27)

380

2708

0.976

7.7

97

n-Octacosane (C28) (Rt Standard 5)

85

2800

   

n -Tetracosanoic acid (24:0) (TMS)

425

2838

0.994

14.7

77

δ-tocopherol (TMS)

474

2900

0.998

17.7

75

n -Nonacosane (C29)

408

2902

0.983

12.2

96

n -Hexacosanol (TMS) (1OH-26:0)

439

2945

0.985

5.1

nd

β-tocopherol (TMS)

488

2987

0.999a

19.8

79

γ-tocopherol (TMS)

488

2999

0.926a

14.1

83

n -Triacontane (C30)

422

3006

0.981

15.3

90

n -Hexacosanoic acid (26:0) (TMS)

468

3037

0.925

9.7

91

n -Heptacosanol (TMS) (1OH-27:0)

453

3043

0.986

10.9

nd

n -Hentriacontane (C31)

436

3105

0.964b

10.1

97

n -Octacosanol (TMS) (1OH-28:0)

467

3139

0.985

11.9

88

α-tocopherol (TMS)

502

3143

0.998c

7.9

82

Cholesterol (TMS)

458

3155

0.983b

13.3

nd

n-Dotriacontane (C32) (Rt Standard 6)

85

3200

   

n -Nonacosanol (TMS) (1OH-29:0)

481

3236

0.917

6.7

nd

Campesterol (TMS)

382

3263

0.989

6.9

102

n -Octacosanoic acid (28:0) (TMS)

481

3269

0.977c

10.5

83

Stigmasterol (TMS)

484

3286

0.989

5.7

92

n -Tritriacontane (C33)

464

3304

0.956b

9.1

91

n -Triacontanol (TMS) (1OH-30:0)

495

3337

0.989

8.8

nd

β-Sitosterol (TMS)

396

3344

0.997

8.1

90

δ-amyrin (TMS)

189

3375

0.993

19.1

nd

β-amyrin (TMS)

498

3385

0.989

17.9

89

n -Triacontanoic acid (30:0) (TMS)

509

3412

0.933c

19.2

76

α-amyrin (TMS)

498

3429

0.992c

15.5

80

n -Tetratriacontane (C34)

478

3463

0.981b

18.2

nd

n -Dotriacontanol (TMS) (1OH-32:0)

523

3533

0.967

9.9

98

n-Hexatriacontane (C36) (RT Standard 7)

85

3600

   
  1. nd – not determined; IS – internal standard
  2. § specific mass ion used for quantification. Full spectrum information could be found at GMD database [55] and in Additional files 1, 2 and 3 (for α-amyrin, δ-amyrin and n-eicosenoic acid)
  3. # relative retention index based on interpolation of retention times between alkane retention standards
  4. a – range 1.25–25 ng injected
  5. b – range 6.25–100 ng injected
  6. c – range 1.25–50 ng injected
Back to article page

Plant Methods

ISSN: 1746-4811