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Figure 1 | Plant Methods

Figure 1

From: Protocol: Streamlined sub-protocols for floral-dip transformation and selection of transformants in Arabidopsis thaliana

Figure 1

Transformation frequencies detected after application of the 'direct dip' protocol. A. tumefaciens strains ABI and GV3101 harboring the CCR2:LUC-HygR transgene were cultured on YEBS or LB liquid media as indicated. Floral dipping of A. thaliana plants was subsequently performed according to the 'direct dip' protocol, involving supplementation of the media with surfactant and sucrose. Selection of T1 seedlings was on solid MS3 medium containing hygromycin.

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